The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis
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چکیده
منابع مشابه
The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis
BACKGROUND The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and others have shown that Bxb1 has catalytic activity in various eukaryotic species including Nicotiana tabacum, Schizosaccharomyces pombe, insects and mammalian cells. RESULTS In this work, the Bxb1 recombinase gene wa...
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BACKGROUND The large serine recombinase phiC31 from broad host range Streptomyces temperate phage, catalyzes the site-specific recombination of two recognition sites that differ in sequence, typically known as attachment sites attB and attP. Previously, we characterized the phiC31 catalytic activity and modes of action in the fission yeast Schizosaccharomyces pombe. RESULTS In this work, the ...
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Vol. 40, No. 4 (2006) BioTechniques 1 Supplementary Figure S1. Structure of constructs used and representation of FRT locus. (A) Plasmids used to detect the Bxb1 integrase-mediated sitespecific recombination. CMVp, SV40t, Luc, stop, and Hygro are cytomegalovirus promoter (CMVp), simian virus 40 terminator (SV40t), luciferase gene, transcription termination sequence, and hygromycin resistance ge...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2012
ISSN: 1472-6750
DOI: 10.1186/1472-6750-12-9